Recombinant Human SEPSECS, His-tagged

• Cat.No.: SEPSECS-931H
• Product Overview: Recombinant human SEPSECS protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 57.9kDa (521aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: SEPSECS, also known as SLA/LP autoantigen, catalyzes the final step of sec synthesis by converting O-phosphoseryl-tRNA(sec) to selenocysteinyl-tRNA(sec) using selenophosphate as the selenium donor. Also, this protein is considered a specific marker of autoimmune hepatitis.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 1mM DTT, 100mM NaCl.
• Purity: > 90% by SDS - PAGE.
• Concentration: 0.5mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MNRESFAAGE RLVSPAYVRQ GCEARRSHEH LIRLL LEKGK CPENGWDEST LELFLHELAI MDSNNFLGNC GVGEREGRVA SALVA RRHYR FIHGIGRSGD ISAVQPKAAG SSLLNKITNS LVLDIIKLAG VHTVANCFVV PMATGMSLTL CFLTLRHKRP KAKYIIWPRI DQKSCFKSMI TAGFEPVVIE NVLEG DELRT DLKAVEAKVQ ELGPDCILCI HSTTSCFAPR VPDRLEELAV ICANYDIPHI VNNAYGVQSS KCMHLIQQGA RVGRIDAFVQ SLDKNFMVPV GGAIIAGFND SFIQE ISKMY PGRASASPSL DVLITLLSLG SNGYKKLLKE RKEMFSYLSN QIKKLSEAYN ERLLHTPHNP ISLAMTLKTL DEHRDKAVTQ LGSMLFTRQV SGARVVPLGS MQTV SGYTFR GFMSHTNNYPCAYLNAASAI GMKMQDVDLF IKRLDRCLKA VRKERS KESD DNYDKTEDVD IEEMALKLDN VLLDTYQDAS S.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Aminoacyl-tRNA biosynthesis

Gene Information

• Gene Name: SEPSECS Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase [ Homo sapiens ]
• Synonyms: Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase; LP; SLA; SLA/LP; MGC161491; DKFZp434B1417; SEPSECS; O-phosphoseryl-tRNA(Sec) selenium transferase; SLA-p35; sec synthase;OTTHUMP00000218475; SLA/LP autoantigen; soluble liver antigen; liver-pancreas antigen; selenocysteine synthase; sep-tRNA:Sec-tRNA synthase; selenocysteinyl-tRNA(Sec) synthase; UGA suppressor tRNA-associated protein; tRNA(Ser/Sec)-associated antigenic protein; soluble liver antigen/liver pancreas antigen; Selenocysteinyl-tRNA(Sec) synthase; Liver-pancreas antigen; Sep-tRNA:Sec-tRNA synthase; Sec synthase; Selenocysteine synthase; Soluble liver antigen; TRNP48; EC 2.9.1.2; SepSecS
• Gene ID: 51091
• mRNA Refseq: NM_016955
• Protein Refseq: NP_058651
• MIM: 613009
• UniProt ID: Q9HD40
• Chromosome Location: 4p15.2
• Function: protein binding; pyridoxal phosphate binding; tRNA binding; transferase activity