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Recombinant Human SERPINB3 Protein, His-tagged

Cat.No.: SERPINB3-506H
Product Overview: Recombinant human SERPINB3 (AAB20405.1) (Asn 2-Pro 390) was expressed, with a polyhistidine tag at the N-terminus.
Source: Baculovirus-Insect Cells
Species: Human
Tag: His
Predicted N Terminal: Met
Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 20% gly, 3mM DTT, 5%~8% trehalose and mannitol.
Bio-activity: Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenice ptide substrate Z-LR-AMC, R&D Systems, Catalog # ES008.The IC50 is
Molecular Mass: The recombinant human SERPINB3 consists of 408 amino acids and predicts a molecular mass of 46.7 kDa. It migrates as an approximately 43 KDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >97 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: SERPINB3 serpin peptidase inhibitor, clade B (ovalbumin), member 3 [ Homo sapiens ]
Official Symbol: SERPINB3
Synonyms: SERPINB3; serpin peptidase inhibitor, clade B (ovalbumin), member 3; SCC, SCCA1, serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3; serpin B3; HsT1196; T4 A; protein T4-A; squamous cell carcinoma antigen 1; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3; SCC; T4-A; SCCA1; SCCA-1; SCCA-PD;
Gene ID: 6317
mRNA Refseq: NM_006919
Protein Refseq: NP_008850
MIM: 600517
UniProt ID: P29508
Chromosome Location: 18q21.3
Pathway: Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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