Recombinant Human SERPINB7, His-tagged

• Cat.No.: SERPINB7-2596H
• Product Overview: Recombinant Human SERPINB7 protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 32-305aa
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.
• Publications:
  Protease-Inhibitor Interaction Predictions: Lessons on the Complexity of Protein–Protein Interactions. (2017)

Gene Information

• Gene Name: SERPINB7 serpin peptidase inhibitor, clade B (ovalbumin), member 7 [ Homo sapiens ]
• Official Symbol: SERPINB7
• Synonyms: SERPINB7; serpin peptidase inhibitor, clade B (ovalbumin), member 7; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 7; serpin B7; MEGSIN; TP55; mesangium predominant gene, megsin; MGC120014; MGC120015; DKFZp686D06190
• Gene ID: 8710
• mRNA Refseq: NM_001040147
• Protein Refseq: NP_001035237
• MIM: 603357
• UniProt ID: O75635
• Chromosome Location: 18q21
• Function: peptidase inhibitor activity; serine-type endopeptidase inhibitor activity

Citation

Protease-Inhibitor Interaction Predictions: Lessons on the Complexity of Protein–Protein Interactions

Journal: Molecular & Cellular Proteomics    Data: 2017/6/1

Authors: Nikolaus Fortelny, Georgina S. Butler, Paul Pavlidis

Article Snippet:Cleavage of quenched fluorescent substrates was measured using excitation/emission wavelengths of 380/460 nm for KLK5 and 320/405 nm for KLK7 as recommended by the suppliers.Cleavage of quenched fluorescent substrates was measured using excitation/emission wavelengths of 380/460 nm for KLK5 and 320/405 nm for KLK7 as recommended by the suppliers.. Serpin B12 was kindly provided by Dr. G. A. Silverman (Children’s Hospital of Pittsburgh); serpin A4 was kindly provided by Dr. J. Chao (Medical University of South Carolina); murine serpin B8 was from Sino Biological Inc. (Catalog# 50215-M08B); and serpin B7 was from Creative BioMart (Catalog# SERPINB7-2596H).. Protease activity was measured after incubation for 1 h at 37 °C with and without serpins in a POLARstar OPTIMA plate reader (BMG Labtech).Protease activity was measured after incubation for 1 h at 37 °C with and without serpins in a POLARstar OPTIMA plate reader (BMG Labtech).