Recombinant Human SHMT1, His-tagged

• Cat.No.: SHMT1-30066TH
• Product Overview: Recombinant full length Human SHMT1 with an N terminal His tag; 503 amino acids with a predicted MWt of 55.2kDa including the tag.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 483 amino acids
• Description: This gene encodes the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. Additional transcript variants have been described, but their biological validity has not been determined.
• Conjugation: HIS
• Molecular Weight: 55.200kDa inclusive of tags
• Form: Liquid
• Purity: >95% by SDS-PAGE
• Storage buffer: pH: 8.00Constituents:0.32% Tris HCl, 10% Glycerol, 0.02% DTT, 0.58% Sodium chloride
• Storage: Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Sequences of amino acids: MGSSHHHHHHSSGLVPRGSHMTMPVNGAHKDADLWSSHDK MLAQPLKDSDVEVYNIIKKESNRQRVGLELIASENFASRA VLEALGSCLNNKYSEGYPGQRYYGGTEFIDELETLCQKRA LQAYKLDPQCWGVNVQPYSGSPANFAVYTALVEPHGRIMG LDLPDGGHLTHGFMTDKKKISATSIFFESMPYKVNPDTGY INYDQLEENARLFHPKLIIAGTSCYSRNLEYARLRKIADE NGAYLMADMAHISGLVAAGVVPSPFEHCHVVTTTTHKTLR GCRAGMIFYRKGVKSVDPKTGKEILYNLESLINSAVFPGL QGGPHNHAIAGVAVALKQAMTLEFKVYQHQVVANCRALSE ALTELGYKIVTGGSDNHLILVDLRSKGTDGGRAEKVLEAC SIACNKNTCPGDRSALRPSGLRLGTPALTSRGLLEKDFQK VAHFIHRGIELTLQIQSDTGVRATLKEFKERLAGDKYQAA VQALREEVESFASFFPLPGLPDF
• Sequence Similarities: Belongs to the SHMT family.

Gene Information

• Gene Name: SHMT1 serine hydroxymethyltransferase 1 (soluble) [ Homo sapiens ]
• Official Symbol: SHMT1
• Synonyms: SHMT1; serine hydroxymethyltransferase 1 (soluble); serine hydroxymethyltransferase, cytosolic; 14 kDa protein; CSHMT; cytoplasmic serine hydroxymethyltransferase; MGC15229; MGC24556; SHMT
• Gene ID: 6470
• mRNA Refseq: NM_004169
• Protein Refseq: NP_004160
• MIM: 182144
• Uniprot ID: P34896
• Chromosome Location: 17p11.2
• Pathway: C1-unit interconversion, eukaryotes, organism-specific biosystem; C1-unit interconversion, eukaryotes, conserved biosystem; Carnitine synthesis, organism-specific biosystem; Cyanoamino acid metabolism, organism-specific biosystem; Cyanoamino acid metabolism, conserved biosystem
• Function: L-allo-threonine aldolase activity; amino acid binding; glycine hydroxymethyltransferase activity; glycine hydroxymethyltransferase activity; protein homodimerization activity