Species : |
Human |
Source : |
HEK293 |
Tag : |
Fc |
Protein Length : |
Lys24-Gly260 |
Tag : |
C-hFc |
Form : |
Lyophilized from 0.22μm filtered solution in 20mM NaAC (pH 5.0). Normally 5% trehalose is added as protectant before lyophilization. |
Bio-activity : |
Immobilized Human Siglec-15, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 22.4ng/ml determined by ELISA. |
Molecular Mass : |
The protein has a predicted MW of 52.6 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result. |
Endotoxin : |
Less than 1EU per μg by the LAL method. |
Purity : |
> 95% as determined by Tris-Bis PAGE |
Storage : |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Reconstitution : |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAC (pH 5.0). |