Recombinant Human SLC8A1 Protein (396-627 aa), His-tagged

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Recombinant Human SLC8A1 Protein (396-627 aa), His-tagged

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Cat.No. : SLC8A1-2278H
Product Overview : Recombinant Human SLC8A1 Protein (396-627 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Partial.
Description : Mediates the exchange of one Ca2+ ion against three to four Na+ ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca2+ levels and Ca2+-dependent cellular processes (PubMed:1374913, PubMed:11241183, PubMed:1476165). Contributes to Ca2+ transport during excitation-contraction coupling in muscle. In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca2+ levels due to release of Ca2+ stores from the endoplasmic reticulum. SLC8A1 mediates the export of Ca2+ from the cell during the next phase, so that cytoplasmic Ca2+ levels rapidly return to baseline. Required for normal embryonic heart development and the onset of heart contractions.
Source : Yeast
Species : Human
Tag : His
Form : Tris-based buffer,50% glycerol
Molecular Mass : 27.4 kDa
Protein length : 396-627 aa
AA Sequence : VNTEVTENDP VSKIFFEQGT YQCLENCGTV ALTIIRRGGD LTNTVFVDFR TEDGTANAGS DYEFTEGTVV FKPGDTQKEI RVGIIDDDIF EEDENFLVHL SNVKVSSEAS EDGILEANHV STLACLGSPS TATVTIFDDD HAGIFTFEEP VTHVSESIGI MEVKVLRTSG ARGNVIVPYK TIEGTARGGG EDFEDTCGEL EFQNDEIVKT ISVKVIDDEE YEKNKTFFLE IG
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA will be sent along with the products. Please refer to it for detailed information.
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Gene Name : SLC8A1 solute carrier family 8 (sodium/calcium exchanger), member 1 [ Homo sapiens ]
Official Symbol : SLC8A1
Synonyms : NCX1;
Gene ID : 6546
mRNA Refseq : NM_021097.2
Protein Refseq : NP_066920.1
MIM : 182305
UniProt ID : P32418

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