Recombinant Human Smad Nuclear Interacting Protein 1, His-tagged

• Cat.No.: SNIP1-5127H
• Product Overview: Recombinant human SNIP1 protein,fused to His-tag at N-terminus, was expressed in E. coli and purified byusing conventional chromatography techniques.

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: SNIP1 is smad nuclear interactingprotein contains a forkhead-associated (FHA) domain and acts as a nuclearinhibitor of CBP/p300. This protein is an inhibitor of the TGF-beta signaltransduction pathway and is important in suppressing transcriptionalactivation dependent on the co-activators CBP and p300. Inhibition ofNF-kappa B activity is a function of the N-terminal domain of SNIP1 andinvolves competition of SNIP1 and the NF-kappa B subunit, RelA/p65, for bindingto p300, similar to the mechanism of inhibition of Smad signaling by SNIP1.
• Concentration: 1 mg/ml
• Form: Suppliedas a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 2mM DTT, 20% glyceroland 100mM NaCl.
• Purity: > 90% by SDS -PAGE
• Sequence: 258-396 amino acids:MGSSHHHHHH SSGLVPRGSH MRWRLYPFKN DEVLPVMYIH RQSAYLLGRH RRIADIPIDH PSCSKQHAVFQYRLVEYTRA DGTVGRRVKP YIIDLGSGNG TFLNNKRIEP QRYYELKEKD VLKFGFSSRE YVLLHESSDTSEIDRKDDED EEEEEEVSDS
• Molecular Mass: 18.8 kDa(160aa), confirmed by MALDI-TOF.
• Applications: SDS-PAGE
• Storage: Storeat 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg Cor - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
• OfficialSymbol: SNIP1
• Pathways: Regulation ofnuclear SMAD2/3 signaling; TGF-beta Receptor Signaling Pathway

Gene Information

• Gene Name: SNIP1 Smad nuclear interactingprotein 1 [ Homo sapiens ]
• Synonyms: SNIP1;Smad nuclear interacting protein 1; FLJ12553;dJ423B22.2; RP3-423B22.3; smad nuclear-interacting protein 1; FHAdomain-containing protein SNIP1; Smad nuclear interacting protein (SNIP1); Smad nuclear-interacting protein 1; OTTHUMP00000004404
• Gene ID: 79753
• mRNA Refseq: NM_024700
• Protein Refseq: NP_078976
• MIM: 608241
• UniProt ID: Q8TAD8
• Chromosome Location: 1p34.3
• Function: proteinbinding