Recombinant Human SMARCA4 Protein, FLAG-tagged

• Cat.No.: SMARCA4-01H
• Product Overview: Full length recombinant human SMARCA4 (BRG1) Remodeling Enzyme produced in SF9 cells.

Specification

• Description: The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Insect Cells
• Species: Human
• Tag: FLAG
• Molecular Mass: 182.7 kDa
• Applications: This product is sufficient to perform 100 remodeling reactions using substrate. A single reaction is defined as 10 μl containing [20 nM SMARCA4/ 20 nM / 2.5 mM rATP] and remodels to completion in <20 minutes. 5x SMARCA Remodeling Assay Buffer is included (100 mM Tris HCl, pH 7.5, 250 mM KCl, 15 mM MgCl2, 0.05% (w/v) BSA, 0.05% (v/v) Tween 20). For best results, make single-use aliquots when first thawed.
• Stability: Stable for six months at -80 centigrade from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
• Concentration: 0.85 mg/ml
• Storage Buffer: 4.3 μl of 20 mM Tris HCl pH 7.9, 150 mM NaCl, 2 mM MgCl2, 0.2 mM EDTA, 10 mM beta-glycerophosphate, 0.01% NP-40, 0.2 mM PMSF, 0.5 mM benzamidine, 1 mM DTT, 15% glycerol, 2 μg/ml leupeptin, 1 μg/ml aprotinin, 0.4 mg/ml recombinant human insulin, 0.4 mg/ml 3x FLAG peptide. Molarity: 4.7 μM

Gene Information

• Gene Name: SMARCA4 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 [ Homo sapiens (human) ]
• Official Symbol: SMARCA4
• Synonyms: SMARCA4; SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4; BRG1; CSS4; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A; SNF2-beta; transcription activator BRG1; ATP-dependent helicase SMARCA4; BRG1-associated factor 190A; BRM/SWI2-related gene 1; SNF2-like 4; brahma protein-like 1; global transcription activator homologous sequence; homeotic gene regulator; mitotic growth and transcription activator; nuclear protein GRB1; protein BRG-1; protein brahma homolog 1; sucrose nonfermenting-like 4
• Gene ID: 6597
• mRNA Refseq: NM_003072
• Protein Refseq: NP_003063
• MIM: 603254
• UniProt ID: P51532