Recombinant Human SMN1, GST-tagged

• Cat.No.: SMN1-169H
• Product Overview: Recombinant Human SMN Tudor Domain (1472-1613 aa) fused to a GST tag at the N-terminus

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 1472-1613 a.a.
• Description: Tudor domains are small protein structural motifs of ~50 amino acids related to the “Royal family” of methyl readers, which also includes chromo, MBT, PWWP, and Agenet-like domains. Tudor domains occur either alone, in tandem, or with other domains and are found in many proteins that are involved in RNA metabolism, germ cell development, transposon silencing, DNA damage response, histone modification and chromatin remodeling. The tudor domains recognize symmetric methylated arginine or methylated lysine residues. The Survival of Motor Neurons (SMN) protein participates in RNA splicing. The Tudor domain of SMN recognizes and binds methylated Sm proteins, which bind small nuclear RNA. SMN is encoded in humans by two separate genes, SMN1 and SMN2, which differ by one base in exon 7. In motor neuron cells, approximately 90% of the SMN2 transcripts are spliced to exclude exon 7. The SMN2 transcripts without exon 7 are less stable than SMN1 transcripts. Consequently, defects in human SMN1 result in the death of motor neuron cells and spinal muscular atrophy, which is the leading genetic cause of infantile death. This protein product contains the tudor domain region of SMN. The sequence of this region is identical in both the SMN1 and the SMN2 genes.
• Form: Liquid. 50 mM Tris-HCl, 150 mM sodium chloride, pH 8.0, containing 20% glycerol.
• Molecular Mass: 42.6 kDa (1472-1613 aa, NT GST Tag)
• Purity: ≥90%
• Storage: Store at -80°C. Avoid repeated freeze and thaw cycles. Stable for ≥6 months

Gene Information

• Gene Name: SMN1 survival of motor neuron 1, telomeric [ Homo sapiens ]
• Official Symbol: SMN1
• Synonyms: SMN1; survival of motor neuron 1, telomeric; SMA, SMA@, spinal muscular atrophy (Werdnig Hoffmann disease, Kugelberg Welander disease); survival motor neuron protein; BCD541; SMA1; SMA2; SMA3; SMNT; gemin 1; gemin-1; component of gems 1; SMA; SMN; SMA4; SMA@; SMN2; T-BCD541
• Gene ID: 6606
• mRNA Refseq: NM_000344
• Protein Refseq: NP_000335
• MIM: 600354
• UniProt ID: Q16637
• Chromosome Location: 5q13.2
• Pathway: Gene Expression, organism-specific biosystem; Metabolism of non-coding RNA, organism-specific biosystem; RNA transport, organism-specific biosystem; RNA transport, conserved biosystem; Survival motor neuron (SMN) complex, organism-specific biosystem; snRNP Assembly, organism-specific biosystem

Citation

A comparison of the performance of voltammetric aptasensors for glycated haemoglobin on different carbon nanomaterials-modified screen printed electrodes.

Journal: Materials science & engineering. C, Materials for biological applications    PubMed ID: 31029337    Data: 2019/8/19

Authors: Shimaa Eissa, Abrar Yousef Almusharraf, Mohammed Zourob

Article Snippet:The aptamer sequences: G15T1 (5’-ACGCACACCAGAGACAAGTAGCCCCCCAAACGCG-3’) and G20T1 (5’-ACACACCCACCCACCAGCCCCAGCATCATGCCCATCCGTCGTGTGT-3’) were custom synthesized by Metabion International (Plangg, Germany).The aptamer sequences: G15T1 (5’-ACGCACACCAGAGACAAGTAGCCCCCCAAACGCG-3’) and G20T1 (5’-ACACACCCACCCACCAGCCCCAGCATCATGCCCATCCGTCGTGTGT-3’) were custom synthesized by Metabion International (Plangg, Germany).. Recombinant human survival motor neuron (SMN) protein was obtained from Creative BioMart (NY, USA). . Recombinant human cystic fibrosis transmembrane conductance regulator (CFTR) protein was purchased from Abcam (MA, USA).Recombinant human cystic fibrosis transmembrane conductance regulator (CFTR) protein was purchased from Abcam (MA, USA).

Truncated aptamers for total and glycated hemoglobin, and their integration into a graphene oxide-based fluorometric method for high-throughput screening for diabetes.

Journal: Mikrochimica acta    PubMed ID: 29675559    Data: 2019/1/29

Authors: Abrar Yousef Almusharraf, Shimaa Eissa, Mohammed Zourob

Article Snippet:Recombinant human cystic fibrosis transmembrane conductance regulator (CFTR) protein was purchased from Abcam (MA, USA) (www.abcam.com/).Recombinant human cystic fibrosis transmembrane conductance regulator (CFTR) protein was purchased from Abcam (MA, USA) (www.abcam.com/).. Recombinant human survival motor neuron (SMN) protein was obtained from Creative BioMart (NY, USA) (www. creativebiomart.net). . The binding buffer composed of 50 mM Tris, pH 7.5, 2 mMMgCl2 and 150 mM NaCl.The binding buffer composed of 50 mM Tris, pH 7.5, 2 mMMgCl2 and 150 mM NaCl.