Recombinant Full Length Human SNAP25 Protein, His tagged

• Cat.No.: SNAP25-296H
• Product Overview: Recombinant full length human SNAP25 (1-206 aa), fused to His tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-206 aa
• Description: Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene.
• Form: Liquid in 50mM HEPES buffer (pH 7.4) containing 5mM DTT, 100mM NaCl
• Molecular Mass: 25.4 kDa
• AASequence: < MGSSHHHHHHSSGLVPRGSH> MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by Bradford assay)
• Publications:
  • Antigen Detection via the Rate of Ion Current Rectification Change of the Antibody-Modified Glass Nanopore Membrane. (2014)

Gene Information

• Gene Name: SNAP25 synaptosome associated protein 25 [ Homo sapiens (human) ]
• Official Symbol: SNAP25
• Synonyms: SNAP25; synaptosome associated protein 25; SUP; RIC4; SEC9; SNAP; CMS18; RIC-4; DEE117; SNAP-25; bA416N4.2; dJ1068F16.2; synaptosomal-associated protein 25; resistance to inhibitors of cholinesterase 4 homolog; synaptosomal-associated protein, 25kDa; synaptosome associated protein 25kDa
• Gene ID: 6616
• mRNA Refseq: NM_130811
• Protein Refseq: NP_570824
• MIM: 600322
• UniProt ID: P60880