Recombinant Human SNF8 Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: SNF8-225H
• Product Overview: SNF8 MS Standard C13 and N15-labeled recombinant protein (NP_009172) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is a component of the endosomal sorting complex required for transport II (ESCRT-II), which regulates the movement of ubiquitinylated transmembrane proteins to the lysosome for degradation. This complex also interacts with the RNA polymerase II elongation factor (ELL) to overcome the repressive effects of ELL on RNA polymerase II activity. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 28.9 kDa
• AA Sequence: MHRRGVGAGAIAKKKLAEAKYKERG TVLAEDQLAQMSKQLDMFKTNLEEF ASKHKQEIRKNPEFRVQFQDMCATI GVDPLASGKGFWSEMLGVGDFYYEL GVQIIEVCLALKHRNGGLITLEELH QQVLKGRGKFAQDVSQDDLIRAIKK LKALGTGFGIIPVGGTYLIQSVPAE LNMDHTVVLQLAEKNGYVTVSEIKA SLKWETERARQVLEHLLKEGLAWLD LQAPGEAHYWLPALFTDLYSQEITA EEAREALPTRTRPLEQKLISEEDLA ANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: SNF8 SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae) [ Homo sapiens (human) ]
• Official Symbol: SNF8
• Synonyms: SNF8; SNF8, ESCRT-II complex subunit, homolog (S. cerevisiae); vacuolar-sorting protein SNF8; Dot3; EAP30; VPS22; hVps22; EAP30 subunit of ELL complex; ESCRT-II complex subunit VPS22; ELL-associated protein of 30 kDa
• Gene ID: 11267
• mRNA Refseq: NM_007241
• Protein Refseq: NP_009172
• MIM: 610904
• UniProt ID: Q96H20