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Recombinant Human SPHK2 Protein, His-tagged

Cat.No. : SPHK2-01H
Product Overview : Recombinant human SPHK2 protein (2-654) with a N-terminal His-tag was expressed in insect cells.
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  • Gene Information
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Description : This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Source : Insect cell
Species : Human
Tag : His
Form : 25 mM HEPES, pH 8.0, with 150 mM sodium chloride, 3 mM DTT, 0.05% Triton X-100, and 5% glycerol
Molecular Mass : 72.9 kDa
Protein length : 2-654
Purity : ≥75% estimated by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to produce 1 µ mol of ADP per minute at 25 centigrade in 20 mM HEPES, pH 7.4, containing 50 mM sodium chloride, 10 mM magnesium chloride, 1 mM EGTA, and 0.02% Triton X-100.
Applications : WB, ELISA, Enzymatic Assay
Stability : ≥ 1 year
Storage : Store at -80 centigrade
Gene Name : SPHK2 sphingosine kinase 2 [ Homo sapiens (human) ]
Official Symbol : SPHK2
Synonyms : SPHK2; sphingosine kinase 2; SK 2; SK-2; SPK 2; SPK-2; sphingosine kinase 2; sphingosine kinase type 2
Gene ID : 56848
mRNA Refseq : NM_001204158
Protein Refseq : NP_001191087
MIM : 607092
UniProt ID : Q9NRA0

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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Can the expression of SPHK2 protein in tissues be measured by immunohistochemistry? 09/23/2021

Immunohistochemistry is a method used to detect the expression level of proteins in tissues. By using antibodies specific to SPHK2 protein, the expression level and distribution of SPHK2 protein in tissues can be detected.

How can the function of SPHK2 protein be studied by gene knockout or overexpression techniques? 06/19/2021

Gene knockout or overexpression techniques can be used to study the function of the SPHK2 protein. By knocking out or overexpressing the SPHK2 gene, the changes in cell growth, differentiation and apoptosis can be observed, and the function of SPHK2 protein in cells can be further revealed.

What is the association between the expression level of SPHK2 protein and disease? 07/02/2020

In some diseases, such as cancer, neurodegenerative diseases, and inflammatory diseases, the expression level of SPHK2 protein may change. High levels of SPHK2 protein may be involved in the pathogenesis and progression of the disease, and may also be a potential target for disease treatment.

What diseases are mutations in the SPHK2 protein associated with? 05/12/2020

Studies have shown that mutations in the SPHK2 protein are associated with cancer, neurodegenerative diseases, and inflammatory diseases. These mutations may lead to dysfunction of proteins, which can lead to the onset and progression of diseases.

How can the function of SPHK2 protein be modulated by pharmacological intervention? 03/04/2020

There are currently no drugs that directly target the SPHK2 protein. However, some drugs, such as kinase inhibitors and anti-inflammatory drugs, can indirectly modulate signal transduction and cellular stress responses. These drugs can improve processes such as cell growth, differentiation, and apoptosis, thereby protecting cells from disease.

How to detect the expression level of SPHK2 gene by PCR? 02/16/2020

The expression level of the SPHK2 gene can be detected using PCR. By designing specific primers for the SPHK2 gene, the expression level of the gene can be amplified and detected.

Customer Reviews (3)

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Reviews
08/18/2022

    SPHK2 is simple to operate and improves the efficiency of the experiment.

    12/29/2021

      The experimental results are reliable and trustworthy.

      03/17/2021

        Easy to purify and operate, easy to store, very good to use.

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