Recombinant Human SPHK2 Protein, His-tagged SPHK2-01H

Recombinant Human SPHK2 Protein, His-tagged

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Recombinant Human SPHK2 Protein, His-tagged

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Cat.No. : SPHK2-01H
Product Overview : Recombinant human SPHK2 protein (2-654) with a N-terminal His-tag was expressed in insect cells.
Description : This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Source : Insect cell
Species : Human
Tag : His
Form : 25 mM HEPES, pH 8.0, with 150 mM sodium chloride, 3 mM DTT, 0.05% Triton X-100, and 5% glycerol
Molecular Mass : 72.9 kDa
Protein length : 2-654
Purity : ≥75% estimated by SDS-PAGE
Unit Definition : One unit is defined as the amount of enzyme required to produce 1 µ mol of ADP per minute at 25 centigrade in 20 mM HEPES, pH 7.4, containing 50 mM sodium chloride, 10 mM magnesium chloride, 1 mM EGTA, and 0.02% Triton X-100.
Applications : WB, ELISA, Enzymatic Assay
Stability : ≥ 1 year
Storage : Store at -80 centigrade
SDS-PAGE Analysis of SPHK2 :

Lane 1: MW Markers, Lane 2: SPHK2 (4 µg), Lane 3: SPHK2 (2 µg). Representative gel image shown; actual purity may vary between each batch.

Figure :

Gene Name : SPHK2 sphingosine kinase 2 [ Homo sapiens (human) ]
Official Symbol : SPHK2
Synonyms : SPHK2; sphingosine kinase 2; SK 2; SK-2; SPK 2; SPK-2; sphingosine kinase 2; sphingosine kinase type 2
Gene ID : 56848
mRNA Refseq : NM_001204158
Protein Refseq : NP_001191087
MIM : 607092
UniProt ID : Q9NRA0

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