Recombinant Human SRP14, His-tagged

• Cat.No.: SRP14-2882H
• Product Overview: Recombinant human SRP14 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: SRP14 is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum. The "Alu domain" of SRP comprises the heterodimer of the SRP9 and SRP14 proteins, which are bound to the 5" and 3" terminal sequences of SRP RNA. SRP9/14 binding may be crucial to the transcription, maturation, nucleolus localization and transport of SRP RNA.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMVLLES EQFLTELTRL FQKCRTSGSV YITLKKYDGR TKPIPKKGTV EGFEPADNKC LLRATDGKKK ISTVVSSKEV NKFQMAYSNL LRANMDGLKK RDKKNKTKKT KAAAAAAAAA PAAAATAATT AATTAATAAQ.
• Purity: > 85% by SDS – PAGE.
• MW: 17.1 kDa (160aa) confirmed by MALDI-TOF.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl, 1mM DTT.
• Concentration: 1mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: SRP14 signal recognition particle 14kDa (homologous Alu RNA binding protein) [ Homo sapiens ]
• Synonyms: SRP14; signal recognition particle 14kDa (homologous Alu RNA binding protein); ALURBP; MGC14326; SRP14; 18 kDa Alu RNA-binding protein; signal recognition particle 14 kDa protein
• Gene ID: 6727
• mRNA Refseq: NM_003134
• Protein Refseq: NP_003125
• MIM: 600708
• UniProt ID: P37108
• Chromosome Location: 15q22
• Pathway: Protein export; Diabetes pathways
• Function: 7S RNA binding; RNA binding; endoplasmic reticulum signal peptide binding; protein binding