Description : |
Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28. Probably by phosphorylating DDX23, leads to the suppression of incorrect R-loops formed during transcription; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA. Can mediate hepatitis B virus (HBV) core protein phosphorylation. Plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles. |
Source : |
HEK293 |
Species : |
Human |
Tag : |
Myc/DDK |
Molecular Mass : |
77.5 kDa |
AA Sequence : |
MSVNSEKSSSSERPEPQQKAPLVPP PPPPPPPPPPPLPDPTPPEPEEEIL GSDDEEQEDPADYCKGGYHPVKIGD LFNGRYHVIRKLGWGHFSTVWLCWD MQGKRFVAMKVVKSAQHYTETALDE IKLLKCVRESDPSDPNKDMVVQLID DFKISGMNGIHVCMVFEVLGHHLLK WIIKSNYQGLPVRCVKSIIRQVLQG LDYLHSKCKIIHTDIKPENILMCVD DAYVRRMAAEATEWQKAGAPPPSGS AVSTAPQQKPIGKISKNKKKKLKKK QKRQAELLEKRLQEIEELEREAERK IIEENITSAAPSNDQDGEYCPEVKL KTTGLEEAAEAETAKDNGEAEDQEE KEDAEKENIEKDEDDVDQELANIDP TWIESPKTNGHIENGPFSLEQQLDD EDDDEEDCPNPEEYNLDEPNAESDY TYSSSYEQFNGELPNGRHKIPESQF PEFSTSLFSGSLEPVACGSVLSEGS PLTEQEESSPSHDRSRTVSASSTGD LPKAKTRAADLLVNPLDPRNADKIR VKIADLGNACWVHKHFTEDIQTRQY RSIEVLIGAGYSTPADIWSTACMAF ELATGDYLFEPHSGEDYSRDEDHIA HIIELLGSIPRHFALSGKYSREFFN RRGELRHITKLKPWSLFDVLVEKYG WPHEDAAQFTDFLIPMLEMVPEKRA SAGECLRHPWLNSSGPTRTRPLEQK LISEEDLAANDILDYKDDDDKV |
Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
Concentration : |
50 μg/mL as determined by BCA |
Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |