Recombinant Human SUCLA2 Protein, His-tagged

• Cat.No.: SUCLA2-001H
• Product Overview: Recombinant Human SUCLA2 protein with a C-terminal His-tag was expressed in E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6.
• Molecular Mass: 29.8 kDa (α), 41.4 kDa (β)
• Purity: ≥85% estimated by SDS-PAGE
• Unit Definition: One unit is defined as the amount of enzyme required to produce 1 nmol NADH per minute at 37 centigradein 34 mM Glycylglycine buffer, pH 8.4, 3.4 mM Magnesium Chloride, 1.2 mM ATP, 0.89 mM Coenzyme A, 5.8 mM Succinic acid, 4.1 U/mL pyruvate kinase, 3.4 U/mL Lactic Dehydrogenase.
• Stability: ≥ 6 months
• Storage: -80 centigrade
• Storage Buffer: 50 mM HEPES, pH 8.0, 150 mM sodium chloride, 20% glycerol

Gene Information

• Gene Name: SUCLA2 succinate-CoA ligase ADP-forming subunit beta [ Homo sapiens (human) ]
• Official Symbol: SUCLA2
• Synonyms: SUCLA2; succinate-CoA ligase ADP-forming subunit beta; A-SCS; A-BETA; MTDPS5; LINC00444; SCS-betaA; succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial; ATP-specific succinyl-CoA synthetase subunit beta; ATP-specific succinyl-CoA synthetase, beta subunit; long intergenic non-protein coding RNA 444; mitochondrial succinyl-CoA ligase [ADP-forming] subunit beta; renal carcinoma antigen NY-REN-39; succinate-CoA ligase ADP-forming beta subunit; succinate-CoA ligase beta subunit; succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial; succinyl-CoA synthetase beta-A chain; EC 6.2.1.5
• Gene ID: 8803
• mRNA Refseq: NM_003850
• Protein Refseq: NP_003841
• MIM: 603921
• UniProt ID: P0A836