Recombinant Human Synaptotagmin I, GST-tagged

• Cat.No.: SYT1-437H
• Product Overview: Recombinant Human synaptotagmin I expressed inE.coliwas cloned with a N-terminal-GST tag and a linker. It consists of the human Synaptotagmin I (residues 96-421, swissprot accession P21579). The GST-tag is cleaved during purification. The protein is mutated in the position I367T and contains a deletion of three amino acids 117A, 118L, and 119K. MW = 37.8 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 96-421 a.a.
• Description: Synaptotagmin-1 is a protein that in humans is encoded by the SYT1 gene. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse.
• Purity: > 95% by SDS-PAGE. The protein was observed as a single band migrating at amolecular weight of (< 45 kDa).
• Supplied As: 1.0mg/ml in PBS pH7.3.The concentration is calculated from the absorbance at 280nm (e280 = 31150 M-1 cm-1).
• Characteristics: Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated to a maximum of 500 μM.
• Storage: -20ºC. The protein is stable at 4ºC for at least 2 weeks and at 25ºC for at least several hours. After initial defrost, aliquot product into individual tubes and refreeze at -20ºC. Avoid repeated freeze/defrost cycles.

Gene Information

• Gene Name: SYT1 synaptotagmin I [ Homo sapiens ]
• Synonyms: SYT1; synaptotagmin I; synaptotagmin 1; P65; SYT; SVP65; DKFZp781D2042; Synaptotagmin-1; DKFZp781D2042; OTTHUMP00000166064; SytI
• Gene ID: 6857
• mRNA Refseq: NM_001135805
• Protein Refseq: NP_001129277
• MIM: 185605
• UniProt ID: P21579
• Chromosome Location: 12cen-q21
• Pathway: Botulinum neurotoxicity; Synaptic Transmission
• Function: calcium ion binding; calcium-dependent protein binding; calmodulin binding; identical protein binding; low-density lipoprotein receptor binding; phosphatidylinositol binding; phospholipid binding; syntaxin-1 binding; transporter activity