Recombinant Human synaptotagmin V, His-tagged

• Cat.No.: SYT5-307H
• Product Overview: SYT5, 109-386aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 109-386 a.a.
• Description: Synaptotagmins, such as SYT5, are a family of type III membrane proteins characterized by cytoplasmic repeats related to protein kinase C regulatory (C2) domains, which are thought to bind calcium. Synaptotagmins may act both as negative regulators of vesicle fusion, allowing fusion in the presence of calcium, and as calcium receptors or sensor molecules. Recombinant human SYT5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl(pH 8.0) containing 40% glycerol, 0.2M NaCl, 2mM DTT
• Molecular Mass: 33.6 kDa (301aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSLGRLQYS LDYDFQSGQL LVGILQAMGL AALDLGGSSD PYVRVYLLPD KRRRYETKVH RQTLNPHFGE TFAFKVPYVE LGGRVLVMAV YDFDRFSRND AIGEVRVPMS SVDLGRPVQA WRELQAAPRE EQEKLGDICF SLRYVPTAGK LTVIVLEAKN LKKMDVGGLS DPYVKVHLLQ GGKKVRKKKT TIKKNTLNPY YNEAFSFEVP CDQVQKVQVE LTVLDYDKLG KNEAIGRVAV GAAAGGAGLR HWADMLANPR RPIAQWHSLR PPDRVRLLPA P
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: SYT5 synaptotagmin V [ Homo sapiens ]
• Official Symbol: SYT5
• Synonyms: SYT5; synaptotagmin V; synaptotagmin-5; synaptotagmin 5; sytV
• Gene ID: 6861
• mRNA Refseq: NM_003180
• Protein Refseq: NP_003171
• MIM: 600782
• UniProt ID: O00445
• Chromosome Location: 19q
• Pathway: Integration of energy metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Regulation of Insulin Secretion, organism-specific biosystem
• Function: metal ion binding; transporter activity