Recombinant Human SYNGAP1 Protein, His-tagged

• Cat.No.: SYNGAP1-2745H
• Product Overview: Recombinant Human SYNGAP1 Protein (Gly443-Asn635) with a N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes a Ras GTPase activating protein that is a member of the N-methyl-D-aspartate receptor complex. The N-terminal domain of the protein contains a Ras-GAP domain, a pleckstrin homology domain, and a C2 domain that may be involved in binding of calcium and phospholipids. The C-terminal domain consists of a ten histidine repeat region, serine and tyrosine phosphorylation sites, and a T/SXV motif required for postsynaptic scaffold protein interaction. The encoded protein negatively regulates Ras, Rap and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor trafficking to the postsynaptic membrane to regulate synaptic plasticity and neuronal homeostasis. Allelic variants of this gene are associated with intellectual disability and autism spectrum disorder. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 25.7 kDa
  Accurate Molecular Mass: 26 kDa
• Protein length: Gly443-Asn635
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: SYNGAP1 synaptic Ras GTPase activating protein 1 [ Homo sapiens (human) ]
• Official Symbol: SYNGAP1
• Synonyms: SYNGAP1; synaptic Ras GTPase activating protein 1; synaptic Ras GTPase activating protein 1 homolog (rat); ras GTPase-activating protein SynGAP; KIAA1938; RASA5; SYNGAP; neuronal RasGAP; synaptic Ras GTPase-activating protein 1; synaptic Ras GTPase activating protein, 135kDa; synaptic Ras GTPase activating protein 1 homolog; MRD5; RASA1; DKFZp761G1421
• Gene ID: 8831
• mRNA Refseq: NM_006772
• Protein Refseq: NP_006763
• MIM: 603384
• UniProt ID: Q96PV0