Recombinant Human T-complex 1, His-tagged

• Cat.No.: TCP1-2851H
• Product Overview: Recombinant human TCP1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. MW = 62.5 kDa (576aa) confirmed by MALDI-TOF.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: TCP1 is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This protein is found in the cytosol as a subunit of a hetero-oligomeric chaperone. It has an important function in maintaining cellular homoeostasis by assisting the folding of many proteins, including the cytoskeletal components actin and tubulin.
• Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEGPLSVFGD RSTGETIRSQ NVMAAASIAN IVKSSLGPVG LDKMLVDDIG DVTITNDGAT ILKLLEVEHP AAKVLCELAD LQDKEVGDGT TSVVIIAAEL LKNADELVKQ KIHPTSVISG YRLACKEAVR YINENLIVNT DELGRDCLIN AAKTSMSSKI IGINGDFFAN MVVDAVLAIK YTDIRGQPRY PVNSVNILKA HGRSQMESML ISGYALNCVV GSQGMPKRIV NAKIACLDFS LQKTKMKLGV QVVITDPEKL DQIRQRESDI TKERIQKILA TGANVILTTG GIDDMCLKYF VEAGAMAVRR VLKRDLKRIA KASGATILST LANLEGEETF EAAMLGQAEE VVQERICDDE LILIKNTKAR TSASIILRGA NDFMCDEMER SLHDALCVVK RVLESKSVVP GGGAVEAALS IYLENYATSM GSREQLAIAE FARSLLVIPN TLAVNAAQDS TDLVAKLRAF HNEAQVNPER KNLKWIGLDL SNGKPRDNKQ AGVFEPTIVK VKSLKFATEA AITILRIDDL IKLHPESKDD KHGSYEDAVH SGALND
• Form: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1 mM DTT, 0.1 mM PMSF,10% Glycerol.
• Purity: > 80% by SDS – PAGE.
• Concentration: 0.5 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: TCP1 t-complex 1 [ Homo sapiens ]
• Synonyms: TCP1; t-complex 1; CCT1; CCTa; D6S230E; CCT-alpha; TCP-1-alpha; T-complex protein 1 subunit alpha; tailless complex polypeptide 1; T-complex protein 1, alpha subunit
• Gene ID: 6950
• mRNA Refseq: NM_001008897
• Protein Refseq: NP_001008897
• MIM: 186980
• UniProt ID: P17987
• Chromosome Location: 6q25.3-q26
• Pathway: Metabolism of proteins
• Function: ATP binding; nucleotide binding; unfolded protein binding