Recombinant Human Tailless Nucleosomes, Biotinylated

• Cat.No.: NUC-006
• Product Overview: Mononucleosomes assembled from recombinant human histones expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4. Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805). All four of the histones have had their tails enzymatically removed after wrapping by 147 base pairs of 601 positioning sequence DNA. There is a 5' biotin-TEG group on the DNA which makes these nucleosomes ideal for use as a negative control in binding assays and pull-down experiments. The nucleosome is the basic subunit of chromatin.
• Form: Purified recombinant tailless nucleosomes (50 µg total mass protein+DNA, 25 µg protein) in 10 mM Tris-HCl pH 7.5, 1 mM EDTA, 25 mM NaCl, 2 mM DTT, & 20% glycerol.
• Applications: ailless Nucleosomes, Human Recombinant, Biotinylated are highly purified and are suitable for use as a negative control or substrate in enzyme screening assays or for nucleosome binding experiments. The absence of histone post-translational modifications makes them ideal for conducting enzyme activity and screening assays. The biotin group on the DNA makes pull-down experiments possible, allowing isolation of the nucleosomes after assay completion.
• Storage: Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.