Recombinant Human TBK1 protein, His-tagged

• Cat.No.: TBK1-337H
• Product Overview: Recombinant Human TBK1(1-729 aa) fused with His tag was expressed in E. coli.

Specification

• Description: The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. This protein is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. For example, the protein can form a complex with the IKB protein TANK and TRAF2 and release the NFKB inhibition caused by TANK.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Tris-based buffer, 50% glycerol
• AA Sequence: MQSTSNHLWL LSDILGQGAT ANVFRGRHKK TGDLFAIKVF NNISFLRPVD VQMREFEVLK KLNHKNIVKL FAIEEETTTR HKVLIMEFCP CGSLYTVLEE PSNAYGLPES EFLIVLRDVV GGMNHLRENG IVHRDIKPGN IMRVIGEDGQ SVYKLTDFGA ARELEDDEQF VSLYGTEEYL HPDMYERAVL RKDHQKKYGA TVDLWSIGVT FYHAATGSLP FRPFEGPRRN KEVMYKIITG KPSGAISGVQ KAENGPIDWS GDMPVSCSLS RGLQVLLTPV LANILEADQE KCWGFDQFFA ETSDILHRMV IHVFSLQQMT AHKIYIHSYN TATIFHELVY KQTKIISSNQ ELIYEGRRLV LEPGRLAQHF PKTTEENPIF VVSREPLNTI GLIYEKISLP KVHPRYDLDG DASMAKAITG VVCYACRIAS TLLLYQELMR KGIRWLIELI KDDYNETVHK KTEVVITLDF CIRNIEKTVK VYEKLMKINL EAAELGEISD IHTKLLRLSS SQGTIETSLQ DIDSRLSPGG SLADAWAHQE GTHPKDRNVE KLQVLLNCMT EIYYQFKKDK AERRLAYNEE QIHKFDKQKL YYHATKAMTH FTDECVKKYE AFLNKSEEWI RKMLHLRKQL LSLTNQCFDI EEEVSKYQEY TNELQETLPQ KMFTASSGIK HTMTPIYPSS NTLVEMTLGM KKLKEEMEGV VKELAENNHI LERFGSLTMD GGLRNVDCL
• Purity: >90% ( SDS-PAGE )
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: Store at -20 centigrade, for extended storage, conserve at -20 centigrade or -80 centigrade.
• Protein length: 1-729

Gene Information

• Gene Name: TBK1 TANK-binding kinase 1 [ Homo sapiens ]
• Official Symbol: TBK1
• Synonyms: TBK1; TANK-binding kinase 1; serine/threonine-protein kinase TBK1; NAK; NF-kB-activating kinase; NF-kappa-B-activating kinase; T2K; FLJ11330
• Gene ID: 29110
• mRNA Refseq: NM_013254
• Protein Refseq: NP_037386
• MIM: 604834
• UniProt ID: Q9UHD2
• Chromosome Location: 12q14.2
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon, organism-specific biosystem; Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; Cytosolic sensors of pathogen-associated DNA, organism-specific biosystem
• Function: ATP binding; nucleic acid binding; nucleotide binding; phosphoprotein binding; protein binding; protein kinase activity; protein serine/threonine kinase activity

Reviews

01/03/2022

Impressed with the quality and swift delivery.

07/27/2020

Five stars for this amazing lab product!

02/20/2019

Top-quality product, superb customer service!

Q&As

What is the significance of TBK1 in the cGAS-STING signaling pathway, and how does it impact immune responses to cytosolic DNA?

TBK1 is a critical component of the cGAS-STING pathway, which senses cytosolic DNA and triggers type I interferon responses. TBK1 phosphorylates STING, initiating downstream signaling events.

How does TBK1 participate in autophagy, and what are the implications for cellular homeostasis?

TBK1 is involved in selective autophagy processes by phosphorylating autophagy receptors and initiating the clearance of damaged organelles or pathogens. This contributes to maintaining cellular homeostasis and responding to stress conditions.

What is the impact of TBK1 on the regulation of inflammation, and how does this influence autoimmune disorders and inflammatory diseases?

TBK1 plays a dual role in inflammation, acting as both a pro-inflammatory and anti-inflammatory mediator. Dysregulation of TBK1 is implicated in autoimmune disorders and inflammatory diseases, highlighting its complex regulatory role.

Can you discuss the role of TBK1 in neurodegenerative diseases, and how dysregulation may contribute to pathogenesis?

TBK1 dysfunction has been associated with neurodegenerative diseases, particularly amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). The precise mechanisms linking TBK1 to these diseases are areas of active research.

Can you elaborate on the molecular mechanisms through which TBK1 regulates type I interferon production?

TBK1 plays a crucial role in the activation of the transcription factor IRF3, leading to the induction of type I interferons. This activation is triggered by the recognition of viral nucleic acids or other pathogen-associated molecular patterns.