Recombinant Human Tetratricopeptide Repeat Domain 1, His-tagged

• Cat.No.: TTC1-6953H
• Product Overview: Recombinant human TTC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specification

• Description: TTC1 is composed of 292 amino acids with three TPR motifs, as a Galpha16-binding protein. The interaction was confirmed both in vitro and in transfected mammalian cells, where TTC1 also binds to several other Galpha proteins. TTC1 was found to interact with Ha-Ras preferentially in its active form.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT.
• Molecular Mass: 36.1 kDa (316aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher).
• Protein length: 1-292aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMGEKSE NCGVPEDLLN GLKVTDTQEA ECAGPPVPDP KNQHSQSKLL RDDEAHLQED QGEEECFHDC SASFEEEPGA DKVENKSNED VNSSELDEEY LIELEKNMSD EEKQKRREES TRLKEEGNEQ FKKGDYIEAE SSYSRALEMC PSCFQKERSI LFSNRAAARM KQDKKEMAIN DCSKAIQLNP SYIRAILRRA ELYEKTDKLD EALEDYKSIL EKDPSIHQAR EACMRLPKQI EERNERLKEE MLGKLKDLGN LVLRPFGLST ENFQIKQDSS TGSYSINFVQ NPNNNR
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TTC1 tetratricopeptide repeat domain 1 [ Homo sapiens ]
• Official Symbol: TTC1
• Synonyms: TTC1; tetratricopeptide repeat domain 1; tetratricopeptide repeat protein 1; TPR1; TPR repeat protein 1; FLJ46404
• Gene ID: 7265
• mRNA Refseq: NM_003314
• Protein Refseq: NP_003305
• MIM: 601963
• UniProt ID: Q99614
• Chromosome Location: 5q32-q33.2
• Function: protein binding; unfolded protein binding