Recombinant Human TFF3, His-tagged

• Cat.No.: TFF3-1046H
• Product Overview: Total 69 AA. MW: 7.82 kDa (calculated). N-terminal His-tag, 10 extra AA(highlighted).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. Human trefoil factor 3 (TFF3, also known as intestinal trefoil factor) belongs together with TFF1 and TFF2 to a small group of mucin-associated peptides. TFF3 contains seven cysteine residues, six of which form disulfide bonds to create a characteristic three-leafed structure. Due to its compact structure, TFF3 is extremely resistant toward acids, proteolytical cleavage or heat degradation.
• Form: Filtered (0.4 μm) and lyophilized from 0.5 mg/ml in 20mM Tris buffer, 150mM NaCl, pH 7.5.
• Research topic: Energy metabolism and body weight regulation, Oncology, Renal disease, Sepsis
• Purity: >95%
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein.
• Sequences of amino acids: MKHHHHHHAS EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Gene Information

• Gene Name: TFF3 trefoil factor 3 (intestinal) [ Homo sapiens ]
• Official Symbol: TFF3
• Synonyms: TFF3; trefoil factor 3 (intestinal); ITF; Polypeptide P1.B; TFI; HITF; trefoil factor 3, HITF, human intestinal trefoil factor; OTTHUMP00000109349; P1B; trefoil factor 3; hITF; hP1.B; Intestinal trefoil factor
• Gene ID: 7033
• mRNA Refseq: NM_003226
• Protein Refseq: NP_003217
• MIM: 600633
• Uniprot ID: Q07654
• Chromosome Location: 21q22.3
• Pathway: HIF-1-alpha transcription factor network, organism-specific biosystem; IL4-mediated signaling events, organism-specific biosystem
• Function: molecular_function