Recombinant Human TGFBI protein, His-tagged

• Cat.No.: TGFBI-553H
• Product Overview: Recombinant Human TGFBI aa. (Gly423~Leu632) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 31kDa as determined by SDS-PAGE reducing conditions
• Protein length: Gly423~Leu632
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.4.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: TGFBI transforming growth factor beta induced [ Homo sapiens (human) ]
• Official Symbol: TGFBI
• Synonyms: TGFBI; transforming growth factor beta induced; CSD; CDB1; CDG2; CSD1; CSD2; CSD3; EBMD; LCD1; BIGH3; CDGG1; transforming growth factor-beta-induced protein ig-h3; RGD-CAP; RGD-containing collagen-associated protein; beta ig-h3; betaig-h3; kerato-epithelin; transforming growth factor beta-induced 68kDa; transforming growth factor, beta-induced, 68kD
• Gene ID: 7045
• mRNA Refseq: NM_000358.2
• Protein Refseq: NP_000349.1
• UniProt ID: Q15582