Species : |
Human |
Source : |
E.coli |
Tag : |
His |
Protein Length : |
Gly423~Leu632 |
Description : |
This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. |
Form : |
Freeze-dried powder |
Molecular Mass : |
31kDa as determined by SDS-PAGE reducing conditions |
Endotoxin : |
<1.0EU per 1µg (determined by the LAL method) |
Purity : |
>95% |
Characteristic : |
The isoelectric point is 9.4. |
Applications : |
SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling. |
Stability : |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Concentration : |
200μg/mL |
Storage buffer : |
PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300. |
Reconstitution : |
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |