Recombinant Human TH protein, His & T7-tagged

• Cat.No.: TH-6962H
• Product Overview: Recombinant Human TH aa. (Leu82~Cys310 (Accession # P07101)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 30.0kDa
• Protein length: Leu82~Cys310 (Accession # P07101)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Characteristic: The isoelectric point is 9.0.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: TH tyrosine hydroxylase [ Homo sapiens (human) ]
• Official Symbol: TH
• Synonyms: TH; tyrosine hydroxylase; TYH; DYT14; DYT5b; tyrosine 3-monooxygenase; dystonia 14; tyrosine 3-hydroxylase
• Gene ID: 7054
• mRNA Refseq: NM_000360.3
• Protein Refseq: NP_000351.2
• UniProt ID: P07101