Recombinant Human Thiamin Pyrophosphokinase 1, His-tagged

• Cat.No.: TPK1-1937H
• Product Overview: Recombinant humanTPK1 protein,fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography.

Specification

• Species: Human
• Tag: His
• Description: TPK1, also known as thiamin pyrophosphokinase 1, catalyzes thephosphorylation of thiamine to thiamine pyrophosphate. This protein can alsocatalyze the phosphorylation of pyrithiamine to pyrithiamine pyrophosphate.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20%glycerol, 1mM DTT.
• Molecular Weight: 29.8 kDa (267aa)confirmed by MALDI-TOF.
• Purity: > 90%by SDS - PAGE
• Concentration: 1 mg/ml (determinedby Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMEHAFT PLEPLLSTGN LKYCLVILNQ PLDNYFRHLW NKALLRACADGGANRLYDIT EGERESFLPE FINGDFDSIR PEVREYYATK GCELISTPDQ DHTDFTKCLK MLQKKIEEKDLKVDVIVTLG GLAGRFDQIM ASVNTLFQAT HITPFPIIII QEESLIYLLQ PGKHRLHVDT GMEGDWCGLIPVGQPCMQVT TTGLKWNLTN DVLAFGTLVS TSNTYDGSGV VTVETDHPLL WTMAIKS
• Storage: Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.
• Pathways: Metabolic pathways; Metabolism;Metabolism of vitamins and cofactors; Metabolism of water-soluble vitaminsand cofactors; Thiamine metabolism; Vitamin B1 (thiamin) metabolism

Gene Information

• Gene Name: TPK1thiamin pyrophosphokinase 1 [ Homo sapiens ]
• Official Symbol: TPK1
• Synonyms: TPK1;thiamin pyrophosphokinase 1; PP20; HTPK1; thiaminpyrophosphokinase 1; thiamine kinase; placental protein 20; thiamine diphosphokinase; Putative uncharacterized protein DKFZp781C0719; DKFZp781C0719
• Gene ID: 27010
• mRNA Refseq: NM_001042482
• Protein Refseq: NP_001035947
• MIM: 606370
• UniProt ID: Q68DW6
• Chromosome Location: 7q34-q35
• Function: ATP binding; kinaseactivity; nucleotide binding; thiamine diphosphokinase activity; transferaseactivity