Recombinant Human Threonyl-tRNA Synthetase, His-tagged

• Cat.No.: TARS-217H
• Product Overview: Recombinant Human PL-7 produced inSf9is a glycosylated, polypeptide chain having a molecular mass of 83,312 dalton. Recombinant PL-7 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Description: Threonyl-tRNA synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 83 kDa protein is an autoantigen recognized by PL-7 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that PL-7 antibodies (similar to Jo-1 antibodies) indicate an increased risk for lung involvement, but this needs to be confirmed for a larger number of cases.
• Physical Appearance: Sterile Filtered clear soultion.
• Formulation: Recombinant Human PL-7 is supplied at a of 0.5 mg/ml in 20mM HEPES buffer pH-8, 200mM sodium chloride, and 20% glycerol.
• Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze-thaw cycles.
• Purity: Greater than 95% (SDS-PAGE).
• Immunological Functions: 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels immuno-dot test).
• Applications: Western-Blot with monoclonal anti-hexa-His-tag antibody & Polymyositis sera.
• Coating Concentration: 0.3-0.8 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.

Gene Information

• Gene Name: TARS threonyl-tRNA synthetase [ Homo sapiens ]
• Synonyms: TARS; threonyl-tRNA synthetase; ThrRS; MGC9344; Threonyl-tRNA synthetase, cytoplasmic; Threonine--tRNA ligase; EC 6.1.1.3; threonine tRNA ligase 1, cytoplasmic
• Gene ID: 1158
• mRNA Refseq: NM_152295
• Protein Refseq: NP_689508
• MIM: 187790
• UniProt ID: P26639
• Chromosome Location: 5p13.2
• Pathway: Aminoacyl-tRNA biosynthesis; Gene Expression
• Function: ATP binding; ligase activity; nucleotide binding; protein binding; protein homodimerization activity; threonine-tRNA ligase activity