Recombinant Human TIE1 protein(Met1-Gln760), His-tagged

• Cat.No.: TIE1-3841H
• Product Overview: Recombinant Human TIE1 (NP_005415.1) (Met 1-Gln 760) was expressed in HEK293, with a C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Met1-Gln760
• Form: Lyophilized from sterile 20 mM Tris, 0.5 M NaCl, 10 % Sucrose, pH 8.2. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant human TIE1 comprises 750 amino acids with a predicted molecular mass of 81.4 kDa. The apparent molecular mass of rhTIE1 is approximately 80-90 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: TIE1 tyrosine kinase with immunoglobulin-like and EGF-like domains 1 [ Homo sapiens ]
• Official Symbol: TIE1
• Synonyms: TIE1; tyrosine kinase with immunoglobulin-like and EGF-like domains 1; TIE, tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1; tyrosine-protein kinase receptor Tie-1; JTK14; tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1; TIE
• Gene ID: 7075
• mRNA Refseq: NM_001253357
• Protein Refseq: NP_001240286
• MIM: 600222
• UniProt ID: P35590