Recombinant Human TIRAP, His-tagged

• Cat.No.: TIRAP-116H
• Product Overview: Recombinant human TIRAP protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-221 a.a.
• Description: The innate immune system recognizes microbial pathogens through Toll-like receptors (TLRs), which identify pathogen-associated molecular patterns. Different TLRs recognize different pathogen-associated molecular patterns and all TLRs have a Toll-interleukin 1 receptor (TIR) domain, which is responsible for signal transduction. The protein encoded by this gene is a TIR adaptor protein involved in the TLR4 signaling pathway of the immune system. It activates NF-kappa-B, MAPK1, MAPK3 and JNK, which then results in cytokine secretion and the inflammatory response. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol,2mM DTT
• Molecular Mass: 26.3 kDa (244aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSSLPPTHASDS GSSRWSKDYD VCVCHSEEDL VAAQDLVSYL EGSTASLRCF LQLRDATPGG AIVSELCQAL SSSHCRVLLI TPGFLQDPWC MASSTSL PAPGSRPKKP LGKMADWFRQ TLLKKPKKRP NSPESTSSDA SQPTSQDSPL PPSLSSVTSP KYQMLQALTE APGAEGCTIP LLSGLSRAAY PPELRFMYYV DGRGPDGGFR QVKEAVMRYL QTLS
• Purity: > 90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: TIRAP toll-interleukin 1 receptor (TIR) domain containing adaptor protein [ Homo sapiens (human) ]
• Official Symbol: TIRAP
• Synonyms: TIRAP; Mal; wyatt; BACTS1; MyD88-2; toll-interleukin 1 receptor (TIR) domain containing adaptor protein; toll/interleukin-1 receptor domain-containing adapter protein; adapter protein wyatt; adaptor protein Wyatt; MyD88 adapter-like protein; Toll-like receptor adaptor protein
• Gene ID: 114609
• mRNA Refseq: NM_001039661
• Protein Refseq: NP_001034750
• MIM: 606252
• UniProt ID: P58753
• Chromosome Location: 11q24.2
• Pathway: AGE/RAGE pathway; Hepatitis B; MyD88:Mal cascade initiated on plasma membrane
• Function: Toll-like receptor 2 binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding, bridging