Recombinant Human TMC8 protein, His-tagged

• Cat.No.: TMC8-3749H
• Product Overview: Recombinant Human TMC8 protein(617-726 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 617-726 aa
• Form: The purified protein was lyophilized from sterile phosphate-buffered saline (PBS: 58 mM Na₂HPO₄, 17 mM NaH₂PO₄, 68 mM NaCl, pH 7.4). Prior to lyophilization, 5% (w/v) trehalose and 5% (w/v) mannitol were incorporated as cryoprotective excipients. The protein was eluted with a buffer containing 300 mM imidazole.
• AASequence: QTQANARAIHRLRKQLVWQVQEKWHLVEDLSRLLPEPGPSDSPGPKYPASQASRPQSFCPGCPCPGSPGHQAPRPGPSVVDAAGLRSPCPGQHGAPASARRFRFPSGAEL
• Purity: 85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Store at 2-8°C for 1-2 weeks. Aliquot and store at -20°C to -80°C for up to 3 months, reconstitution with sterile water and addition of an equal volume of glycerol. Avoid repeat freeze-thaw cycles.
• Reconstitution: Dissolve the product in 200 μl sterile water to achieve a working concentration of 0.25 µg/μl. If experimental compatibility allows, mix the aqueous solution with an equal volume of glycerol (1:1 ratio) after initial reconstitution.

Gene Information

• Gene Name: TMC8 transmembrane channel-like 8 [ Homo sapiens ]
• Official Symbol: TMC8
• Synonyms: TMC8; transmembrane channel-like 8; epidermodysplasia verruciformis 2 , EVER2; transmembrane channel-like protein 8; EVIN2; epidermodysplasia verruciformis 2; epidermodysplasia verruciformis protein 2; EV2; EVER2; FLJ40668; FLJ43684; MGC40121; MGC102701
• Gene ID: 147138
• mRNA Refseq: NM_152468
• Protein Refseq: NP_689681
• MIM: 605829
• UniProt ID: Q8IU68