Recombinant Human TRNA Aspartic Acid Methyltransferase 1, His-tagged

• Cat.No.: TRDMT1-5583H
• Product Overview: Recombinant humanTRDMT1 protein, fused to His-tag at N-terminus, wasexpressed in E. coli and purified by using conventionalchromatography.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: TRDMT1 also known asDNMT2, specificallymethylates cytosine 38 in the anticodon loop of tRNA (Asp).
• Form: Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT.
• Molecular Weight: 47.2 kDa (415aa)confirmed by MALDI-TOF
• Purity: > 90% by SDS-PAGE
• Concentration: 1 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMEPLRV LELYSGVGGM HHALRESCIP AQVVAAIDVN TVANEVYKYNFPHTQLLAKT IEGITLEEFD RLSFDMILMS PPCQPFTRIG RQGDMTDSRT NSFLYILDIL PRLQKLPKYILLENVKGFEV SSTRDLLIQT IENCGFQYQE FLLSPTSLGI PNSRLRYFLI AKLQSEPLPF QAPGQVLMEFPKIESVHPQK YAMDVENKIQ EKNVEPNISF DGSIQCSGKD AILFKLETAE EIHRKNQQDS DLSVKMLKDFLEDDTDVNQY LLPPKSLLRY ALLLDIVQPT CRRSVCFTKG YGSYIEGTGS VLQTAEDVQV ENIYKSLTNLSQEEQITKLL ILKLRYFTPK EIANLLGFPP EFGFPEKITV KQRYRLLGNS LNVHVVAKLI KILYE
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• OfficialSymbol: TRDMT1
• Pathway: Cysteine and methioninemetabolism; Metabolic pathways

Gene Information

• Gene Name: TRDMT1 tRNA aspartic acidmethyltransferase 1 [ Homo sapiens ]
• Synonyms: TRDMT1; tRNAaspartic acid methyltransferase 1; DMNT2; DNMT2; PuMet; RNMT1; M.HsaIIP; DNAmethyltransferase-2; DNA MTase homolog HsaIIP; DNA methyltransferase homologHsaIIP; DNA (cytosine-5-)-methyltransferase 2; EC 2.1.1.29; DNA(cytosine-5)-methyltransferase-like protein; MHSAIIP; PUMET; OTTHUMP00000019228;tRNA (cytosine(38)-C(5))-methyltransferase; tRNA(cytosine-5-)-methyltransferase; EC 2.1.1
• Gene ID: 1787
• mRNA Refseq: NM_004412
• Protein Refseq: NP_004403
• MIM: 602478
• UniProt ID: O14717
• Chromosome Location: 10p15.5