Recombinant Human TTK protein, His-tagged

• Cat.No.: TTK-392H
• Product Overview: Recombinant Human TTK(Gly532~Leu786) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: This gene encodes a dual specificity protein kinase with the ability to phosphorylate tyrosine, serine and threonine. Associated with cell proliferation, this protein is essential for chromosome alignment at the centromere during mitosis and is required for centrosome duplication. It has been found to be a critical mitotic checkpoint protein for accurate segregation of chromosomes during mitosis. Tumorigenesis may occur when this protein fails to degrade and produces excess centrosomes resulting in aberrant mitotic spindles. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Molecular Mass: 33.3kDa
• Protein length: Gly532~Leu786
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 95%
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: TTK TTK protein kinase [ Homo sapiens ]
• Official Symbol: TTK
• Synonyms: TTK; TTK protein kinase; dual specificity protein kinase TTK; cancer/testis antigen 96; CT96; MPS1L1; monopolar spindle 1-like 1; phosphotyrosine picked threonine kinase; phosphotyrosine picked threonine-protein kinase; ESK; PYT; MPS1; FLJ38280
• Gene ID: 7272
• mRNA Refseq: NM_003318
• Protein Refseq: NP_003309
• MIM: 604092
• UniProt ID: P33981
• Chromosome Location: 6q13-q21
• Pathway: Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem
• Function: ATP binding; binding; nucleotide binding; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; protein tyrosine kinase activity