Recombinant Human Type II Topoisomerase, His-tagged

• Cat.No.: TOP2B-01H
• Product Overview: Recombinant hTOP2βcore (residues 445-1201), fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 445-1201 a.a.
• Form: 20 mM Tris-HCl, 200 mM NaCl, pH7.4
• Molecular Mass: ~87 kDa
• AA Sequence: SVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIME NAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIV KASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAF SKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRK VLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASP RYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRR MLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPAL ISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLS YYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEDET QNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAFVEELDKV ESQERED
• Purity: >92% as determined by SDS-PAGE
• Storage: Short Term Storage at +4°C. Long Term Storage at -20°C to -80°C. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: TOP2B topoisomerase (DNA) II beta 180kDa [ Homo sapiens ]
• Official Symbol: TOP2B
• Synonyms: TOP2B; topoisomerase (DNA) II beta 180kDa; topoisomerase (DNA) II beta (180kD); DNA topoisomerase 2-beta; topo II beta; antigen MLAA-44; topoisomerase IIb; topoisomerase II beta; U937 associated antigen; DNA topoisomerase II beta; DNA topoisomerase II, 180 kD; DNA topoisomerase II, beta isozyme; TOPIIB; top2beta
• Gene ID: 7155
• mRNA Refseq: NM_001068
• Protein Refseq: NP_001059
• MIM: 126431
• UniProt ID: Q02880
• Chromosome Location: 3p24
• Function: ATP binding; DNA topoisomerase (ATP-hydrolyzing) activity; DNA-dependent ATPase activity; chromatin binding; enzyme binding; histone deacetylase binding; isomerase activity; nucleotide binding; protein C-terminus binding; protein heterodimerization activity; protein kinase C binding