Recombinant Human UBA52 protein, His & T7-tagged

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Recombinant Human UBA52 protein, His & T7-tagged

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Cat.No. : UBA52-1246H
Product Overview : Recombinant Human UBA52 aa. (Met1~Lys128 (Accession # P62987) fused with N-terminal His & T7 tag was produced in E. coli cells.
Description : Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome.
Source : E. coli
Species : Human
Tag : His & T7
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 18.4kDa.
Protein length : Met1~Lys128 (Accession # P62987)
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : >90%
Characteristic : The isoelectric point is 9.9.
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
SDS-PAGE :

Gene Name : UBA52 ubiquitin A-52 residue ribosomal protein fusion product 1 [ Homo sapiens (human) ]
Official Symbol : UBA52
Synonyms : UBA52; ubiquitin A-52 residue ribosomal protein fusion product 1; L40; CEP52; RPL40; HUBCEP52; ubiquitin-60S ribosomal protein L40; ubiquitin carboxyl extension protein 52; ubiquitin-52 amino acid fusion protein; ubiquitin-CEP52
Gene ID : 7311
mRNA Refseq : NM_001033930.2
Protein Refseq : NP_001029102.1
UniProt ID : P62987

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