Recombinant Human UBE2B Protein (S2-S152), Tag Free

• Cat.No.: UBE2B-0019H
• Product Overview: Recombinant Human (GG)-UBE2B(S2-S152 end) Protein was expressed in Insect cell.

Specification

• Description: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.
• Source: Insect cell
• Species: Human
• Tag: No tag
• Form: Liquid
• Protein Length: S2-S152
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 85%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50mM Tris-HCl (pH 7.5), 200mM NaCl, 20% glycerol, 1mM DTT.
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: UBE2B ubiquitin-conjugating enzyme E2B [ Homo sapiens (human) ]
• Official Symbol: UBE2B
• Synonyms: UBE2B; ubiquitin-conjugating enzyme E2B; ubiquitin conjugating enzyme E2B (RAD6 homolog); ubiquitin-conjugating enzyme E2 B; HHR6B; RAD6B; UBC2; E2 protein; RAD6 homolog B; ubiquitin-protein ligase B; ubiquitin carrier protein B; ubiquitin-conjugating enzyme E2-17 kDa; ubiquitin-conjugating enzyme E2B (RAD6 homolog); HR6B; E2-17kDa
• Gene ID: 7320
• mRNA Refseq: NM_003337
• Protein Refseq: NP_003328
• MIM: 179095
• UniProt ID: P63146