Recombinant Human UBE2H protein, GST-tagged

• Cat.No.: UBE2H-375H
• Product Overview: Recombinant Human UBE2H protein (Met1-Leu183), fused to GST tag at N-terminus, was expressed in E.coli.

Specification

• Description: Ubiquitin-conjugating enzyme E2 H (UBE2H), a member of the ubiquitin-conjugating enzyme family, is also known as ubiquitin carrier protein H, ubiquitin-conjugating enzyme E2-20K and ubiquitin-protein ligase H. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. At the same time, ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. Furthermore,UBE2H can accept ubiquitin from the E1 complex and catalyze its covalent attachment to other proteins. In vitro,UBE2H can aslo catalyze 'Lys-11'- and 'Lys-48'-linked polyubiquitination, and it is capable, in vitro, to ubiquitinate histone H2A.
• Source: E.coli
• Species: Human
• Tag: GST
• Form: Lyophilized from 0.22 um filtered solution in 50 mM HEPES, 150 mM NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 47.6 kDa. The protein migrates as 45 kDa under reducing (R) condition (SDS-PAGE).
• Protein length: 183
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: UBE2H
• Official Symbol: UBE2H
• Synonyms: UBE2H; ubiquitin-conjugating enzyme E2H; ubiquitin conjugating enzyme E2H (homologous to yeast UBC8) , ubiquitin conjugating enzyme E2H (UBC8 homolog, yeast); ubiquitin-conjugating enzyme E2 H; UBC8; UBCH; ubiquitin-protein ligase H; ubiquitin carrier protein H; ubiquitin-conjugating enzyme E2-20K; ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast); ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8); UBCH2; E2-20K
• Gene ID: 7328
• mRNA Refseq: NM_001202498
• Protein Refseq: NP_001189427
• MIM: 601082
• UniProt ID: P62256