Recombinant Human UBE2J1, GST-tagged

• Cat.No.: UBE2J1-126H
• Product Overview: Recombinant full-length human UBE2J1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: UBE2J1 is a member of the E2 ubiquitin-conjugating enzyme family which is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system. UBE2J1 is play an important role in the homeostatic regulation of MHC class I assembly and expression. HRD1 and UBE2J1 associate in a complex together with non-β2m bound MHC class I heavy chains, Derlin 1, and p97 and discriminate misfolded MHC class I from conformational MHC I-β2m-peptide heterotrimers.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~70 kDa
• Purity: >70% by densitometry
• Applications: Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: UBE2J1 ubiquitin-conjugating enzyme E2, J1, U [ Homo sapiens ]
• Official Symbol: UBE2J1
• Synonyms: UBE2J1; ubiquitin-conjugating enzyme E2, J1, U; ubiquitin conjugating enzyme E2, J1 (UBC6 homolog, yeast); ubiquitin-conjugating enzyme E2 J1; CGI 76; HSPC153; NCUBE1; UBC6; HSUBC6e; non-canonical ubiquitin-conjugating enzyme 1; yeast ubiquitin-conjugating enzyme UBC6 homolog E; ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast); Ubc6p; CGI-76; HSPC205; NCUBE-1; HSU93243; MGC12555
• Gene ID: 51465
• mRNA Refseq: NM_016021
• Protein Refseq: NP_057105
• UniProt ID: Q9Y385
• Chromosome Location: 6q16.1
• Pathway: Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination & Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Parkinsons disease, organism-specific biosystem
• Function: ATP binding; acid-amino acid ligase activity; ligase activity; nucleotide binding; ubiquitin-protein ligase activity