Recombinant Human UBE3A Protein, C-His tagged (Isoform I)

• Cat.No.: UBE3A-35H
• Product Overview: Recombinant Human UBE3A Protein (Isoform I) with C-His tag was expressed in HEK293.

Specification

• Description: This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.
• Source: HEK293
• Species: Human
• Tag: C-His
• Molecular Mass: 99 kDa
• AA Sequence: MKRAAAKHLIERYYHQLTEGCGNEA CTNEFCASCPTFLRMDNNAAAIKAL ELYKINAKLCDPHPSKKGASSAYLE NSKGAPNNSCSEIKMNKKGARIDFK DVTYLTEEKVYEILELCREREDYSP LIRVIGRVFSSAEALVQSFRKVKQH TKEELKSLQAKDEDKDEDEKEKAAC SAAAMEEDSEASSSRIGDSSQGDNN LQKLGPDDVSVDIDAIRRVYTRLLS NEKIETAFLNALVYLSPNVECDLTY HNVYSRDPNYLNLFIIVMENRNLHS PEYLEMALPLFCKAMSKLPLAAQGK LIRLWSKYNADQIRRMMETFQQLIT YKVISNEFNSRNLVNDDDAIVAASK CLKMVYYANVVGGEVDTNHNEEDDE EPIPESSELTLQELLGEERRNKKGP RVDPLETELGVKTLDCRKPLIPFEE FINEPLNEVLEMDKDYTFFKVETEN KFSFMTCPFILNAVTKNLGLYYDNR IRMYSERRITVLYSLVQGQQLNPYL RLKVRRDHIIDDALVRLEMIAMENP ADLKKQLYVEFEGEQGVDEGGVSKE FFQLVVEEIFNPDIGMFTYDESTKL FWFNPSSFETEGQFTLIGIVLGLAI YNNCILDVHFPMVVYRKLMGKKGTF RDLGDSHPVLYQSLKDLLEYEGNVE DDMMITFQISQTDLFGNPMMYDLKE NGDKIPITNENRKEFVNLYSDYILN KSVEKQFKAFRRGFHMVTNESPLKY LFRPEEIELLICGSRNLDFQALEET TEYDGGYTRDSVLIREFWEIVHSFT DEQKRLFLQFTTGTDRAPVGGLGKL KMIIAKNGPDTERLPTSHTCFNVLL LPEYSSKEKLKERLLKAITYAKGFG MLHHHHHHHH
• Purity: > 90% by SDS-PAGE
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.57 mg/mL by BCA
• Storage Buffer: PBS, pH7.4

Gene Information

• Gene Name: UBE3A ubiquitin protein ligase E3A [ Homo sapiens (human) ]
• Official Symbol: UBE3A
• Synonyms: UBE3A; ubiquitin protein ligase E3A; AS; ANCR; PIX1; E6-AP; HPVE6A; EPVE6AP; ubiquitin-protein ligase E3A; CTCL tumor antigen se37-2; E6AP ubiquitin-protein ligase; HECT-type ubiquitin transferase E3A; human papilloma virus E6-associated protein; human papillomavirus E6-associated protein; oncogenic protein-associated protein E6-AP; renal carcinoma antigen NY-REN-54; EC 2.3.2.26
• Gene ID: 7337
• mRNA Refseq: NM_000462
• Protein Refseq: NP_000453
• MIM: 601623
• UniProt ID: Q05086