Recombinant Human UBK0 Protein, His-tagged

• Cat.No.: UBK0-411H
• Product Overview: Recombinant Human UBK0 Protein is produced by Bacteria expression system. This protein is fused with a His tag.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 µM. In 6xHis-Ub(K0), all lysine residues were substituted by arginines, thereby it can only support monoubiquitination, but not polyubiquitin chains.
• Form: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
• Molecular Mass: 8.5 kDa
• Purity: > 95 % by SDS-PAGE
• Storage: Store at -80 centigrade; avoid multiple freeze-thaw cycles.

Gene Information

• Synonyms: Ubiquitin (K0)