Recombinant Human Unc-51-Like Kinase 3 (C. elegans), His-tagged

• Cat.No.: ULK3-1116H
• Product Overview: Full-length recombinant human ULK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Specification

• Species: Human
• Source: Sf10 Insect Cell
• Tag: His
• Description: ULK3 or unc-51-like kinase 3 is a member of the serine/threonine kinase family that is involved in the SHH signaling pathway as a positive regulator of GLI proteins. ULK3 enhances endogenous and over-expressed GLI transcriptional activity in cultured cells and alters its subcellular localization. Furthermore, ULK3 phosphorylates GLI proteins in vitro. ULK3 is widely expressed and its expression is higher in a number of tissues where SHH signaling is known to be active, again suggesting that ULK3 is involved in the SHH pathway as a positive regulator of GLI proteins.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 51kDa
• Expression System: Sf9 insect cells using baculovirus
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
• Specific Activity: 230nmol/min/mg
• Purity: >90%
• Concentration: 0.1 ug/ul
• Sequences: Full-length
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Product shipped on dry ice.
• Pathways: Regulation of autophagy; mTOR signaling pathway

Gene Information

• Gene Name: ULK3 unc-51-like kinase 3 (C. elegans) [ Homo sapiens ]
• Official Symbol: UKL3
• Synonyms: ULK3; unc-51-like kinase 3 (C. elegans); FLJ90566; DKFZp434C131; serine/threonine-protein kinase ULK3; DKFZP434C131; EC 2.7.11.1; Unc-51-like kinase 3
• Gene ID: 25989
• mRNA Refseq: NM_001099436
• Protein Refseq: NP_001092906
• MIM: 613472
• UniProt ID: Q6PHR2
• Chromosome Location: 15q24.1
• Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity