Recombinant Human unconventional SNARE in the ER 1 homolog (S. cerevisiae), His-tagged

• Cat.No.: USE1-584H
• Product Overview: USE1, 1-231aa, Human, His tag, E.coli

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-231 a.a.
• Description: USE1, as known as P31, is a vesicle transport protein. This protein is a component of a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L and SEC22B. It interacts directly with STX18. SNARE may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. Recombinant human USE1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol 0.1M NaCl, 1mM DTT
• Molecular Mass: 28.3kDa (254aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAASRLE LNLVRLLSRC EAMAAEKRDP DEWRLEKYVG ALEDMLQALK VHASKPASEV INEYSWKVDF LKGMLQAEKL TSSSEKALAN QFLAPGRVPT TARERVPATK TVHLQSRARY TSEMRSELLG TDSAEPEMDV RKRTGVAGSQ PVSEKQSAAE LDLVLQRHQN LQEKLAEEML GLARSLKTNT LAAQSVIKKD NQTLSHSLKM ADQNLEKLKT ESERLEQHTQ KSVN
• Purity: >80% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: USE1 unconventional SNARE in the ER 1 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: USE1
• Synonyms: P31; SLT1; MDS032
• Gene ID: 55850
• mRNA Refseq: NM_018467.3
• Protein Refseq: NP_060937.1
• MIM: 610675
• UniProt ID: Q9NZ43
• Chromosome Location: 19p13.11
• Pathway: SNARE interactions in vesicular transport, organism-specific biosystem;SNARE interactions in vesicular transport, conserved biosystem
• Function: protein binding