Recombinant Human UOX Protein

• Cat.No.: UOX-01H
• Product Overview: Recombinant Human UOX Protein was expressed in E.coli cell.

Specification

• Description: The cDNA coding for urate-oxidase was cloned from a strain of Aspergillus flavus . The monomer protein has no intra- or
  inter-disulfide bridges. Urate oxidase catalyzes the enzymatic oxidation (degrades) of uric acid into allantoin, an inactive and
  soluble metabolite, which is 5 to 10 fold more soluble than uric acid . Urate oxidase is an enzyme of the purine breakdown
  pathway that catalyses the oxidation of uric acid to allantoin. Uricase is present in numerous diverse organisms, but not in
  higher primates including human. Hyperuricaemia is most commonly associated with gout and also occurs in mammalians with
  malignancy, especially those with lymphoid malignancies due to rapid cell turnover and an increased rate of purine metabolism.
  Uricase is effective in the prevention and treatment of hyperuricaemia in mammalians with malignancy and in those who have
  undergone transplantation. It appears to act rapidly, safely and induces a more dramatic decrease in plasma levels of uric acid.
• Source: Escherichia coli
• Species: Human
• Form: Lyophilized
• Molecular Mass: 34.247 kDa
• Protein Length: 302 amino acids
• AA Sequence: msavkaaryg kdnvrvykvh kdektgvqtv yemtvcvlle geietsytka dnsvivatds ikntiyitak qnpvtppelf gsilgthfie
  kynhihaahv nivchrwtrm didgkphphs firdseekrn vqvdvvegkg idiksslsgl tvlkstnsqf wgflrdeytt lketwdrils
  tdvdatwqwk nfsglqevrs hvpkfdatwa tarevtlktf aednsasvqa tmykmaeqil arqqlietve yslpnkhyfe
  idlswhkglq ntgknaevfa pqsdpnglik ctvgrsslks kl
• Purity: Greater than 96.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE
• Storage: Lyophilized Urate Oxidase although stable at room temperature for 3 weeks, should be stored
  desiccated below -18°C. Upon reconstitution Uricase should be stored at 4°C between 2-7 days
  and for future use below -18°C.Please prevent freeze-thaw cycles
• Storage Buffer: Each 1.5mg Uricase contains 5mg sucrose, 25mg glycine, 0.1mg Tween-80, 13.6 mg
  Na2HPO4*12H20 and 0.33 mg NaH2PO4*2H20.
• Reconstitution: We highly recommend reconstituting the lyophilized Uricase in 50mM borate buffer containing 0.001%Triton X-100 and 1.0mM
  EDTA, pH 8.5 for activity assay. The specific activity was found to be 10U/mg.One Unit oxidizes one micromole of uric acid per
  minute at 25°C, at pH 8.5.

Gene Information

• Gene Name: UOX urate oxidase (pseudogene) [ Homo sapiens (human) ]
• Official Symbol: UOX
• Synonyms: Urate Oxidase,Uricase,Urate Oxygen,Oxidoreductase,UOX,UO,EC 1.7.3.
• Gene ID: 391051