Active Recombinant Human UPRT protein, His-GST-tagged

• Cat.No.: UPRT-1435H
• Product Overview: Active Recombinant Human UPRT protein(Q96BW1)(75-297 aa), fused with N-terminal His tag and GST tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST&His
• Protein Length: 75-297 aa
• Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: Uracil Phosphoribosyltransferase (UPRT) is an important enzyme involved in the pyrimidine salvage pathway, which is a critical metabolic pathway for the synthesis of nucleotides in various organisms, including humans. This enzyme plays a key role in the conversion of uracil, a pyrimidine base, into uridine-5'-phosphate (UMP), a precursor for the synthesis of RNA and DNA. Besides, Purine Nucleoside Phosphorylase (PNP) has been identified as an interactor of UPRT, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human UPRT and recombinant mouse PNP. Briefly, UPRT was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PNP-coated microtiter wells and incubated for 1h at 37°C. Wells were washed with PBST and incubated for 1h with anti-UPRT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37°C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human UPRT and recombinant mouse PNP was shown, the EC50 for this effect is 0.017 ug/mL.
• Molecular Mass: 54.7kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 90%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: UPRT uracil phosphoribosyltransferase (FUR1) homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: UPRT
• Synonyms: UPRT; uracil phosphoribosyltransferase (FUR1) homolog (S. cerevisiae); uracil phosphoribosyltransferase homolog; DKFZp781E1243; FUR1; MGC23937; RP11 311P8.3; UPRTase; UMP pyrophosphorylase; UPP; RP11-311P8.3
• Gene ID: 139596
• mRNA Refseq: NM_145052
• Protein Refseq: NP_659489
• MIM: 300656
• UniProt ID: Q96BW1