Recombinant Human USP3 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: USP3-2633H
• Product Overview: USP3 MS Standard C13 and N15-labeled recombinant protein (NP_006528) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 59.3 kDa
• AA Sequence: MECPHLSSSVCIAPDSAKFPNGSPS SWCCSVCRSNKSPWVCLTCSSVHCG RYVNGHAKKHYEDAQVPLTNHKKSE KQDKVQHTVCMDCSSYSTYCYRCDD FVVNDTKLGLVQKVREHLQNLENSA FTADRHKKRKLLENSTLNSKLLKVN GSTTAICATGLRNLGNTCFMNAILQ SLSNIEQFCCYFKELPAVELRNGKT AGRRTYHTRSQGDNNVSLVEEFRKT LCALWQGSQTAFSPESLFYVVWKIM PNFRGYQQQDAHEFMRYLLDHLHLE LQGGFNGVSRSAILQENSTLSASNK CCINGASTVVTAIFGGILQNEVNCL ICGTESRKFDPFLDLSLDIPSQFRS KRSKNQENGPVCSLRDCLRSFTDLE ELDETELYMCHKCKKKQKSTKKFWI QKLPKVLCLHLKRFHWTAYLRNKVD TYVEFPLRGLDMKCYLLEPENSGPE SCLYDLAAVVVHHGSGVGSGHYTAY ATHEGRWFHFNDSTVTLTDEETVVK AKAYILFYVEHQAKAGSDKLTRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: USP3 ubiquitin specific peptidase 3 [ Homo sapiens (human) ]
• Official Symbol: USP3
• Synonyms: USP3; ubiquitin specific peptidase 3; ubiquitin specific protease 3; ubiquitin carboxyl-terminal hydrolase 3; ubiquitin thioesterase 3; deubiquitinating enzyme 3; ubiquitin thiolesterase 3; ubiquitin-specific-processing protease 3; UBP; SIH003; MGC129878; MGC129879
• Gene ID: 9960
• mRNA Refseq: NM_006537
• Protein Refseq: NP_006528
• MIM: 604728
• UniProt ID: Q9Y6I4