Recombinant Human USP40 overexpression lysate, Myc/DDK-tagged
Cat.No. : | USP40-1001HCL |
Product Overview : | Transient overexpression lysate of ubiquitin specific peptidase 40 (USP40) with a C-Myc/DDK tag was expressed in HEK293T. HEK293T cells in 10-cm dishes were transiently transfected with cDNA plasmid. Transfected cells were cultured for 48 hrs before collection. The cells were lysed in modified RIPA buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1 mM EDTA, 1 x Proteinase inhibitor cocktail mix, 1 mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20 centigrade before shipping. Components: 1 vial of 100 μg gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 μg empty vector transfected control cell lysate in RIPA buffer 1 vial of 250 μL 2 x SDS Sample Buffer (4% SDS, 125 mM Tris-HCl pH 6.8, 10% Glycerol, 0.002% Bromphenol blue, 100 mM DTT) |
- Specification
- Gene Information
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Description : | Modification of cellular proteins by ubiquitin is an essential regulatory mechanism controlled by the coordinated action of multiple ubiquitin-conjugating and deubiquitinating enzymes. USP40 belongs to a large family of cysteine proteases that function as deubiquitinating enzymes. |
Source : | HEK293T |
Species : | Human |
Tag : | Myc/DDK |
Molecular Mass : | 141.4 kDa |
Storage : | Upon receiving, store the sample at -80 centigrade. Also after dilution, the protein sample should be aliquoted and stored at -80 centigrade for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2 x SDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80 centigrade. |
Shipping : | Dry ice |
Gene Name : | USP40 ubiquitin specific peptidase 40 [ Homo sapiens (human) ] |
Official Symbol : | USP40 |
Synonyms : | USP40; ubiquitin specific peptidase 40; ubiquitin carboxyl-terminal hydrolase 40; deubiquitinating enzyme 40; ubiquitin specific protease 40; ubiquitin thioesterase 40; ubiquitin thiolesterase 40; ubiquitin-specific-processing protease 40; EC 3.4.19.12 |
Gene ID : | 55230 |
mRNA Refseq : | NM_018218 |
Protein Refseq : | NP_060688 |
MIM : | 610570 |
UniProt ID : | Q9NVE5 |
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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