Recombinant Human VEGFC protein, His-Avi-tagged

• Cat.No.: VEGFC-4633H
• Product Overview: Recombinant Human VEGFC protein(Q6FH59)(Thr103-Arg227), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Thr103-Arg227
• Tag: C-His-Avi
• Form: Lyophilized from 0.22μm filtered solution in 50mM MES, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human VEGF-C, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 17.9ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 17.1 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM MES, 150mM NaCI (pH 6.0).

Gene Information

• Gene Name: VEGFC vascular endothelial growth factor C [ Homo sapiens ]
• Official Symbol: VEGFC
• Synonyms: VEGFC; vascular endothelial growth factor C; VRP; VEGF-C; FLT4 ligand DHM; vascular endothelial growth factor-related protein; Flt4-L
• Gene ID: 7424
• mRNA Refseq: NM_005429
• Protein Refseq: NP_005420
• MIM: 601528
• UniProt ID: P49767