Recombinant Human VIT, His-tagged

• Cat.No.: VIT-3663H
• Product Overview: Recombinant Human VIT protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-351aa
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.
• Publications:
  Nanoparticle-protein complexes mimicking corona formation in ocular environment (2016)
  Studies on Regulation of Retinal and Choroidal Neovascularization via Intravitreal Injection of Nanoparticles (2016)

Gene Information

• Gene Name: VIT vitrin [ Homo sapiens ]
• Official Symbol: VIT
• Synonyms: VIT; vitrin; MGC70561; MGC149746; DKFZp313L1517
• Gene ID: 5212
• mRNA Refseq: NM_001177969
• Protein Refseq: NP_001171440
• UniProt ID: Q6UXI7
• Chromosome Location: 2p22.2

Citation

Nanoparticle-protein complexes mimicking corona formation in ocular environment.

Journal: Biomaterials    PubMed ID: 27648757    Data: 2018/5/31

Authors: Dong Hyun Jo, Jin Hyoung Kim, Jeong Hun Kim

Article Snippet:To investigate the biological consequences of nanoparticleprotein complexes, we primed nanoparticles (1 109 particles) with the top 5 corona proteins including SFRP2 (50 ng, cat. no. 6838-FR-025/CF, R&D), albumin (25 ng, cat. no. A9731-1G, Sigma), vitrin (25 ng, cat. no. VIT-3663H, Creative BioMart) , alpha-crystallin A chain (25 ng, cat. no. 15,152, Cayman), and RBP3 (25 ng, cat. no. RPU143618, Biomatik) for 1 h. After centrifugation and resuspension, the nanoparticle-protein complexes were utilized for further analyses including VEGF-binding assay, tube formation assay, cell viability assay, and in vivo laser-induced photocoagulation model in mice.