Recombinant Human VPS35 293 Cell Lysate VPS35-389HCL

Recombinant Human VPS35 293 Cell Lysate

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Cat.No. : VPS35-389HCL Optional Service: Optional requirements on this protein
Description : Antigen standard for vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : VPS35 vacuolar protein sorting 35 homolog (S. cerevisiae) [ Homo sapiens ]
Official Symbol : VPS35
Synonyms : VPS35; vacuolar protein sorting 35 homolog (S. cerevisiae); vacuolar protein sorting 35 (yeast homolog) , vacuolar protein sorting 35 (yeast); vacuolar protein sorting-associated protein 35; FLJ10752; MEM3; hVPS35; maternal-embryonic 3; PARK17; FLJ13588; FLJ20388; DKFZp434E1211; DKFZp434P1672;
Gene ID : 55737
mRNA Refseq : NM_018206
Protein Refseq : NP_060676
MIM : 601501
UniProt ID : Q96QK1
Chromosome Location : 16q12
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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