Recombinant Human VSIR protein, Avi & His-tagged, Biotinylated

• Cat.No.: VSIR-1289H
• Product Overview: Biotinylated Human B7-H5 is expressed from human 293 cells (HEK293). It contains AA Phe 33 - Ala 194 (Accession # AAH20568.1). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: Phe 33 - Ala 194
• Description: Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.
• Predicted N Terminal: Phe 33
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 20.8 kDa. As a result of glycosylation, the protein migrates as 35-45 kDa under reducing (R) condition, and 35-45 kDa under non-reducing (NR) condition (SDS-PAGE).
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please avoid repeated freeze-thaw cycles.
  No activity loss is observed after storage at:
  4-8°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Gene Name: VSIR V-set immunoregulatory receptor [ Homo sapiens (human) ]
• Official Symbol: VSIR
• Synonyms: B7H5; GI24; B7-H5; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha; Death Domain1alpha; PDCD1 homolog; V-domain Ig suppressor of T cell activation; V-set domain-containing immunoregulatory receptor; platelet receptor GI24; sisp-1; stress-induced secreted protein-1; V-type immunoglobulin domain-containing suppressor of T-cell activation
• Gene ID: 64115
• mRNA Refseq: NM_022153.1
• Protein Refseq: NP_071436.1
• UniProt ID: Q9H7M9