Active Recombinant Human VSIR protein, His-tagged

• Cat.No.: VSIR-19H
• Product Overview: Active Recombinant Human VSIR protein(Q9H7M9)(Phe33-Ala194) is expressed from HEK293 with His tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 33-194 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human B7-H5, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-B7-H5 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 19 kDa. Due to glycosylation, the protein migrates to 32-70 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 1 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.