"VSIR" Related Products

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Recombinant Human VSIR protein, His-tagged

Cat.No. : VSIR-19H
Product Overview : Recombinant Human VSIR protein (Phe33-Ala194), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).
Description : Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.
Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 20.0 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : 162
Endotoxin : Less than 1.0 EU per ug by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of VSIR-19H :

Human B7-H5, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR of VSIR-19H :

Captured Human VSIG3, Fc Tag on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human B7-H5, His Tag with an affinity constant of 31.5 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI of VSIR-19H :

Loaded Human VSIG3, Fc Tag on AHC Biosensor, can bind Human B7-H5, His Tag with an affinity constant of 16 μM as determined in BLI assay (Routinely tested).

Gene Name : VSIR
Official Symbol : VSIR
Synonyms : B7H5; GI24; B7-H5; Dies1; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha
Gene ID : 64115
mRNA Refseq : NM_022153.2
Protein Refseq : NP_071436.1
MIM : 615608
UniProt ID : Q9H7M9

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